ISO 10315:2021 Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas-chromatographic method

标准简介

Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas-chromatographic methodWARNING — The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and determine the applicability of any other restrictions prior to use.是国际标准化组织(International Organization for Standardization，简称ISO)于2021-06发布的ISO标准，适用于国际范围。

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标准文档类型为Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas-chromatographic methodWARNING — The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and determine the applicability of any other restrictions prior to use.高清PDF版本（文字版），标准文档内可进行搜索，可以复制原文，可粘贴。

标准部分原文

Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas-

chromatographic method

WARNING — The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and determine the applicability of any other restrictions prior to use.

1 Scope

This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISO 4387.

NOTE ISO 20778 and ISO 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

2 Normative references

The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 4387, Cigarettes — Determination of total and nicotine-free dry particulate matter using a routine analytical smoking machine

3 Terms a nd definiti ons

No terms and definitions are listed in this document.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— ISO Online browsing platform: available at https:// www. iso .org/ obp

— IEC Electropedia: available at https:// www.e lectropedia .org/ 4 Principle

The total particulate matter from the mainstream smoke, which shall be obtained in accordance with ISO 4387, is dissolved in an extraction solution containing an internal standard. The nicotine content of an aliquot of the smoke extract is determined by gas chromatography, and the nicotine content in the total particulate matter from the mainstream smoke is calculated.

NOTE In countries not in a position to use the gas-chromatographic method, reference is made to ISO 3400 for the determination of total nicotine alkaloids. In such cases, values obtained using the method described in ISO 3400 can be used with the addition of a note in the expression of results.

5 Reagents

Use only reagents of recognized analytical reagent grade.

5.1 Carrier gas, helium (CAS: 7440-59-7), nitrogen (CAS: 7727-37-9) or hydrogen (CAS: 1333-74-0) of high purity.

5.2 Auxiliary gases, hydrogen (CAS: 1333-74-0) of high purity and air for the flame ionization detector.

5.3 Propan-2-ol (CAS: 67-63-0), with maximum water content of 1,0 mg/ml.

5.4 Internal standard, n-heptadecane (CAS: 629-78-7) or quinaldine (CAS: 91-63-4) of purity not less than 99 %.

Carvone (CAS: 99-49-0), n-octadecane (CAS: 593-45-3), or other appropriate internal standards may be used after assessment of their purity and determination that the internal standard does not co-elute with other components in the smoke extract. The peak area of the internal standard in smoke extracts should be monitored for consistency. In cases where inconsistencies are found, analysis of an extraction of a smoke sample without the internal standard in the extraction solution should be performed to confirm the absence of a peak in the smoke extract eluting at the same time as the internal standard (see Clause 10).

5.5 Extraction solution, propan-2-ol (5.3) containing an appropriate concentration of internal standard (5.4); this is normally in the range of 0,2 mg/ml to 0,5 mg/ml.

Solution not stored in a temperature-controlled laboratory shall be allowed to equilibrate to ambient temperature before use.

5.6 Reference substance, nicotine (CAS: 54-11-5) of known purity not less than 98 %.

Store this between 0 °C and 4 °C and exclude light.

Nicotine salicylate (CAS: 29790-52-1) of known purity not less than 98 % may also be used.

The purity of the nicotine or nicotine salicylate may be verified in accordance with ISO 13276 or by any other validated method.

5.7 Calibration solutions

Dissolve the nicotine (5.6) in the extraction solution (5.5) to produce a series of at least four calibration solutions with concentrations that cover the range expected to be found in the test portion (usually 0,02 mg/ml to 2,0 mg/ml). Store these solutions at between 0 °C and 4 °C and exclude light.

Solutions stored at low temperatures shall be allowed to equilibrate to ambient temperature before use.

6 Apparatus

Usual laboratory apparatus and, in particular, the following items.

6.1 Gas-chromatograph, equipped with a flame ionization detector and a suitable data handling instrument (see Clause 10).

6.2 Column, of internal diameter between 2 mm and 4 mm and preferably of length 1,5 m to 2 m.

The column is preferably made of glass, but other materials such as deactivated stainless steel or nickel may be used. Stationary phase: 10 % poly(ethylene glycol) (PEG) 20 000 plus 2 % potassium hydroxide on an acid-washed silanized support material, 150 µm (100 mesh) to 190 µm (80 mesh) (see also Clause 10).

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